Osmosis in Red Onion Cells

Osmosis in Red Onion Cells By Youssef Gharib apprise Description of Osmosis in Red Onion jail cellphones Osmosis is the diffusion of water from an subject field of low concentration to an ara of high concentration across a semi-permeable membrane. The purpose of this lab is to compare the three different sheaths of final results affect on the relative surface of the vacuole to the cell, the outmost membrane of onion plant ruddy cells (tunics) are used to figure out the different types. In the fierce onion you can see effects promptly when the onion cells are located in different type of firmness of purposes.The effect of the solutions is shown through the color patch within the membrane since thats where the vacuoles are present. Question How will the vacuole within(a) the cells of the outer membrane of a red onion react when deionized water, a 1%, and a 10% saline solution solution are added to the cells? system If a saline solution is added to the outer membran e of a red onion cell, then based on the count of the solution, it will progress to the vacuole inside the cell to become hypertonic thus ricocheting in sizing.This is because water moves from inside the vacuole, to the cell since the saline solution is entering the vacuole. season if deionized water is added on to cell, then the vacuole size should get larger or stay the same. Variables In hooked Variables type of solution, amount of solution, and the type of onion. Levels of IV Deionized water, 1% saline solution, 10% saline solution, red onion outer membrane. checker group Red onion without any solution added to it. Dependent Variables amount of water inside the vacuole. Controlled Variables . Constant temperature this will be insured by keeping the room at a invariable temperature of 21 degrees, because if temperature is not kept constant, the selective information might be stirred. 2. Type of onion this will be insured by using the same onion (red onion) to involve d ata, if a different type of onion is used, this might cause the data to be inaccurate. 3. Microscope while collecting data, and observing what happens to the red onion, the same microscope shall be used for observing what happens to the vacuoles. Materials * Red Onion Scissors/ Scalpel * Microscope * 1%, 10% saline solutions * Deionized/Distilled water * Plastic cups * Cover slips Procedure 1. Take a small piece of onion and peel off a rag week of the purple undress. Cut a piece of skin about the size of a little fingernail. 2. Place the piece onto a microscope slide, and put it under the microscope to drop data as this is the image group. 3. Then add louvre drops of deionized water onto the slide, then record qualitative and vicenary observations on the size of the vacuole inside the cell. 4.Then repeat step one, and put the piece of skin on another microscope slide. 5. Add five drops of 1% saline solution on the slide, then record qualitative and quantitative observations on the alternate in size of the onion cell vacuole. 6. Step four was iterate, then five drops of 10% saline solution were dropped onto the slide. Record qualitative and quantitative observations again on the change in size of the onion cell vacuole. 7. Steps 2, 3, 5, and 6 were all save under high-power aim in the microscope. 8. Clean and dry slide and coverslip.Data/Data hold over The effect of Deionized water, 1%, and 10% saline solution on the outer membrane of a red onion Amount of water inside the vacuoles No solution (control group) Deionized water 1% solution 10% solution Before snow% 100% 100% 100% After 100% 100% 85% 65% % change 0% 0% 15% 35% Qualitative data/ Observations No change, purple onion cell walls scarce visible No change, deionized water didnt cause vacuoles to shrink Vacuoles shrank, but not significant amount. Vacuoles shrank a lot, and are visibly hypertonic Data AnalysisIn the lab, observations were recorded to see how a 1% saline solution and a 10% saline solution affected the size of the vacuole inside the cells of the outer membrane of a red onion. The case-by-case variables in the essay were the type of solution, amount of solution, and the type of onion. There was a 1% saline solution and a 10% saline solution. The type of onion used in this test was a red onion, and the purple outer membrane was used to record data. The control group that the experiment was based upon was a slice of the outer membrane without any solutions added to it.The dependent variable was the amount of water inside the vacuole that changed based on the add-on of the saline solutions, and the deionized water. The controlled variables in the experiment were constant temperature, the type of onion, and the microscope. The constant temperature would be insured by keeping the room at a constant temperature of 21 degrees (room temp. ), because if temperature is not kept constant, the data would be affected. The type of onion will be insured by using th e same onion (red onion) to collect data, if a different type of onion is used, this would increase circumstances of wrongful conduct while collecting data.While collecting data, and observing what happens to the red onion, the same microscope was used for observing what happens to the vacuoles. The data collected and observations recorded showed that the more(prenominal) concentrated the saline solutions were, the more the vacuole shrunk in size. For example, when the 1% solution was added, only 15% of the vacuole bemused water, while when the 10% solution was added, 35% of the vacuole lost water. The deionized water however showed barely any change in size, so it did not shrink or grow.The percentages recorded in the data hedge though were not accurate since they were just assumptions because the tools to correctly measure the percent change were not present. Since the experiment was only conducted once, there were no repeated trials, leading the data to be more prone to err ors and uncertainties. other error in the data was that at first many slices of the tunic had to be peeled to finally get a mavin strand of cells, because every time a slice would be peeled, there would be a double layer of cells, cause it to be hard to see through the microscope.Conclusion and Evaluation In the lab, many conclusions were drawn from the results found. The data calculated, and the observations recorded supported the hypothesis, since in the data the saline solutions caused the vacuoles to shrink, and the deionized water caused the vacuole to stay the same. The saline solutions caused the vacuoles to shrink because since there was more salt outside the vacuole, the vacuole became hypertonic causing it to release water and absorb salt into the vacuole.The use of a control group in this experiment establishes the basis of a means for comparison between the different levels of IV and the control group. The procedure was not as descriptive as possible, and could arri ve been more explanatory, and instructive. L fictives in the experiment could have been that the tunic of the red onion was not thin enough to record accurate data since it was very hard to get a single strand of cells without having two strands on top of each other. Another imitation in the experiment wouldve been that the correct tools to measure the quantitative data in the experiment were not present, and it caused the data to be approximated, and not accurate. A weakness in the experiment could have been that at first, the saline solutions didnt reckon to change the size of the vacuoles, until another observation was conducted from the microscope and it became visibly different. An error in the experiment wouldve included only having one trial, and not repeating any.Another error couldve been contaminations with the contact of the cells with skin, and or contaminations with the dropper used for the experiment. Improvements that couldve been made to this experiment were repeat ed trials to make the data more precise, also a commission to measure the relative size of the vacuole to the cell more accurately. Another proceeds couldve been using a microscope with higher magnification and resolution leading to interrupt image quality and better data. Protocol Diagram